pSIM24-GUS
(Plasmid #67882)

• Purpose: Plant Gene Expression Vector pSIM24 containing the GUS (Beta-glucuronidase) reporter gene
• Depositing Lab: Indu Maiti
• Publication: Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988. eCollection 2014.

Backbone

• Vector backbone: pSIM24
• Backbone size w/o insert (bp): 7081
• Vector type: Plant Expression
• Selectable markers: Kanamycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: uidA
• Alt name: β-glucuronidase
• Alt name: GUS
• Species: E. coli
• Mutation: T120A
• GenBank ID: NC_000913.3
• Promoter: M24

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: SstI (not destroyed)
• 5′ sequencing primer: NOS-F (5'-GCGTTCAAAAGTCGCCTAAG-3')
• 3′ sequencing primer: NOSterm-R (5'-attgccaaatgtttgaacga-3')

Resource Information

• Supplemental Documents:
  • pSIM24 Genbank File

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC NGS identifies a T120A discrepancy in the GUS insert. It is unknown what, if any, impact this may have on plasmid function.