Codon Optimized Dysferlin
(Plasmid #67878)

• Purpose: The human dysferlin cDNA was codon optimized in an attempt to increase Dysferlin production in human cells.
• Depositing Lab: Matthew Hirsch
• Publication: Hirsch et al Mol Ther. 2013 Dec;21(12):2205-16. doi: 10.1038/mt.2013.184. Epub 2013 Aug 13.

Backbone

• Vector backbone: unknown
• Backbone manufacturer: genscript
• Backbone size w/o insert (bp): 2940
• Total vector size (bp): 9180
• Vector type: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: codon optimized Dysferlin
• Species: Synthetic
• Insert Size (bp): 6240
• Promoter: No Promoter
• Tag / Fusion Protein:
  • No

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GGT ACCTCGCGAA TGCATC
• 3′ sequencing primer: CATATGGCGG CCGCGTCGAC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: synthesized by genscript
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry