p123shy1-3HA
(Plasmid #67876)

• Purpose: encodes shy1 with a C-terminal 3xHA-tag under control of its endogenous promoter.
• Depositing Lab: Armin Djamei
• Publication: Rabe et al Mol Microbiol. 2013 Jul;89(1):179-88. doi: 10.1111/mmi.12269. Epub 2013 Jun 11.

Backbone

• Vector backbone: p123
• Vector type: expression in Ustilago maydis
• Selectable markers: Carboxin resistance for selection in Ustilago maydis.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: shy1
• Alt name: um05230
• Species: U. maydis
• Insert Size (bp): 1330
• Promoter: shy1
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pBRforEco
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • p123shy1-3HA genbank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

By linearizing it in the CBX gene it can be used for integration into the ip-locus of U.maydis.