pRSET-GST-05230
(Plasmid #67874)

• Purpose: E.coli expression vector derived from pRSET encoding shy1 (um05230) with a N-terminal GST-fusion.
• Depositing Lab: Armin Djamei
• Publication: Rabe et al Mol Microbiol. 2013 Jul;89(1):179-88. doi: 10.1111/mmi.12269. Epub 2013 Jun 11.

Backbone

• Vector backbone: pRSET-GST
• Backbone manufacturer: Djamei lab
• Modifications to backbone: Added GST to pRSET vector
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: shy1
• Alt name: um05230
• Species: U. maydis
• Insert Size (bp): 1330
• Promoter: T7
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspHI/NcoI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pGEX5
• 3′ sequencing primer: T7 Terminal

Resource Information

• Supplemental Documents:
  • pRSET-GST-05230 genbank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry