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Addgene

pET28-At_PRORP2-His
(Plasmid #67870)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67870 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28-b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PRORP2
  • Alt name
    At2g16650
  • Species
    A. thaliana (mustard weed)
  • GenBank ID
    816166
  • Entrez Gene
    PRORP2 (a.k.a. AT2G16650, T24I21.6, T24I21_6, proteinaceous RNase P 2)
  • Promoter T7
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-At_PRORP2-His was a gift from Walter Rossmanith (Addgene plasmid # 67870 ; http://n2t.net/addgene:67870 ; RRID:Addgene_67870)

  • For your References section:

    tRNA processing by protein-only versus RNA-based RNase P: kinetic analysis reveals mechanistic differences. Pavlova LV, Gossringer M, Weber C, Buzet A, Rossmanith W, Hartmann RK. Chembiochem. 2012 Oct 15;13(15):2270-6. doi: 10.1002/cbic.201200434. Epub 2012 Sep 13. 10.1002/cbic.201200434 PubMed 22976545
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