pET28-MRPP3-His
(Plasmid #67866)

• Purpose: bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag
• Depositing Lab: Walter Rossmanith
• Publication: Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583-93. doi: 10.1093/nar/gks910. Epub 2012 Oct 5.

Backbone

• Vector backbone: pET28-b(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MRPP3
• Alt name: PRORP
• Alt name: KIAA0391
• Species: H. sapiens (human)
• Insert Size (bp): 1698
• Mutation: Amino acid 46 of recombinant MRPP3 is preceded by Met-Gly
• GenBank ID: 9692
• Entrez Gene: KIAA0391 (a.k.a. MRPP3, PRORP)
• Promoter: T7
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• Supplemental Documents:
  • pET28-MRPP3-His sequence file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry