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pET21-MRPP1_native+
(Plasmid #67865)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67865 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-21b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5442
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MRPP1
  • Alt name
    TRMT10C
  • Alt name
    RG9MTD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1095
  • Mutation
    mitoch. form (contains aa 40-403)
  • GenBank ID
    54931
  • Entrez Gene
    TRMT10C (a.k.a. COXPD30, HNYA, MRPP1, RG9MTD1)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21-MRPP1_native+ was a gift from Walter Rossmanith (Addgene plasmid # 67865 ; http://n2t.net/addgene:67865 ; RRID:Addgene_67865)

  • For your References section:

    A subcomplex of human mitochondrial RNase P is a bifunctional methyltransferase--extensive moonlighting in mitochondrial tRNA biogenesis. Vilardo E, Nachbagauer C, Buzet A, Taschner A, Holzmann J, Rossmanith W. Nucleic Acids Res. 2012 Dec;40(22):11583-93. doi: 10.1093/nar/gks910. Epub 2012 Oct 5. 10.1093/nar/gks910 PubMed 23042678
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