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Addgene

pET28-HADH2
(Plasmid #67863)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67863 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28-b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HADH2
  • Alt name
    MRPP2
  • Alt name
    SDR5C1
  • Alt name
    HSD17B10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    846
  • GenBank ID
    3028
  • Entrez Gene
    HSD17B10 (a.k.a. 17b-HSD10, ABAD, CAMR, DUPXp11.22, ERAB, HADH2, HCD2, HSD10MD, MHBD, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-HADH2 was a gift from Walter Rossmanith (Addgene plasmid # 67863 ; http://n2t.net/addgene:67863 ; RRID:Addgene_67863)

  • For your References section:

    RNase P without RNA: identification and functional reconstitution of the human mitochondrial tRNA processing enzyme. Holzmann J, Frank P, Loffler E, Bennett KL, Gerner C, Rossmanith W. Cell. 2008 Oct 31;135(3):462-74. doi: 10.1016/j.cell.2008.09.013. 10.1016/j.cell.2008.09.013 PubMed 18984158
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