GFP-V96 ELP
(Plasmid #67853)

• Purpose: GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues
• Depositing Lab: Andrew MacKay
• Publication: Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):3439-44. doi: 10.1021/bm301090x. Epub 2012 Oct 25.

Backbone

• Vector backbone: NT-GFPpcDNA3.1
• Backbone size w/o insert (bp): 5256
• Total vector size (bp): 7556
• Modifications to backbone: Refer to plasmid construction paper. (NT-GFPpcDNA3.1 (Invitrogen) was modified to contain a BsgI restriction site downstream of the GFP gene)
• Vector type: Mammalian Expression
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GFP fused to ELP (VPGVG) repeated 96 times
• Alt name: GFP-V96
• Species: H. sapiens (human)
• Insert Size (bp): 2200
• Promoter: T7
• Tag / Fusion Protein:
  • GFP fused to N-terminal of ELP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsgI (unknown if destroyed)
• 3′ cloning site: Pme1 (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGHrev

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry