N-TAP A2A in pcDNA3
(Plasmid
#67850)
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Purposemammalian expression of A2A receptor with N terminal TAP tag
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67850 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.0
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameadenosine A2a receptor
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SpeciesH. sapiens (human)
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Entrez GeneADORA2A (a.k.a. A2aR, ADORA2, RDC8)
- Promoter CMV
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Tag
/ Fusion Protein
- TAP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
N-TAP A2A in pcDNA3 was a gift from Christian Gruber (Addgene plasmid # 67850 ; http://n2t.net/addgene:67850 ; RRID:Addgene_67850) -
For your References section:
Recruitment of a cytoplasmic chaperone relay by the A2A adenosine receptor. Bergmayr C, Thurner P, Keuerleber S, Kudlacek O, Nanoff C, Freissmuth M, Gruber CW. J Biol Chem. 2013 Oct 4;288(40):28831-44. doi: 10.1074/jbc.M113.464776. Epub 2013 Aug 21. 10.1074/jbc.M113.464776 PubMed 23965991