pMito-rxRFP1.1
(Plasmid #67841)

• Purpose: redox-sensitive red fluorescent protein rxRFP1.1, mitochondrially localized
• Depositing Lab: Huiwang Ai
• Publication: Fan et al Anal Bioanal Chem. 2016 Jan 12.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: rxRFP1.1 and mitochondrial localization sequence
• Species: Synthetic
• Insert Size (bp): 1003
• Promoter: CMV
• Tags / Fusion Proteins:
  • mitochondrial localization sequence (N terminal on insert)
  • His Tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV forward

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry