pBAD-rxRFP1.2
(Plasmid
#67839)
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Purposeredox-sensitive red fluorescent protein rxRFP1.2
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67839 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAD/His B
- Backbone size w/o insert (bp) 4100
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerxRFP1.2
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SpeciesSynthetic
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Insert Size (bp)903
- Promoter pBAD
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Tag
/ Fusion Protein
- His Tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xho I (not destroyed)
- 3′ cloning site Hind III (not destroyed)
- 5′ sequencing primer pBAD Forward
- 3′ sequencing primer pBAD Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD-rxRFP1.2 was a gift from Huiwang Ai (Addgene plasmid # 67839 ; http://n2t.net/addgene:67839 ; RRID:Addgene_67839) -
For your References section:
Development of redox-sensitive red fluorescent proteins for imaging redox dynamics in cellular compartments. Fan Y, Ai HW. Anal Bioanal Chem. 2016 Jan 12. 10.1007/s00216-015-9280-3 [pii] PubMed 26758595