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Addgene

pcDNA3-pHRFP
(Plasmid #67832)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67832 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pH sensitive red fluorescent protein pHRFP
  • Alt name
    pHRFP
  • Species
    Synthetic
  • Insert Size (bp)
    916
  • GenBank ID
    KM658165
  • Promoter CMV
  • Tag / Fusion Protein
    • His Tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pHRFP insert contains I279V and T295S compared to closest reference sequence AJB84631.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-pHRFP was a gift from Huiwang Ai (Addgene plasmid # 67832 ; http://n2t.net/addgene:67832 ; RRID:Addgene_67832)

  • For your References section:

    Monitoring redox dynamics in living cells with a redox-sensitive red fluorescent protein. Fan Y, Chen Z, Ai HW. Anal Chem. 2015 Mar 3;87(5):2802-10. doi: 10.1021/ac5041988. Epub 2015 Feb 20. 10.1021/ac5041988 PubMed 25666702
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