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Addgene

pERB257
(Plasmid #67761)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67761 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEM791
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KLC1-mCherry-eDHFR
  • Species
    R. norvegicus (rat)
  • Mutation
    contains Klc1 amino acids 1-175
  • GenBank ID
    NM_001081973.1 NP_001075442.1
  • Entrez Gene
    Klc1 (a.k.a. Kns2)
  • Promoter CAG
  • Tags / Fusion Proteins
    • mCherry (C terminal on insert)
    • eDHFR (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
  • 3′ sequencing primer Bglob-pA-R (5'-ttttggcagagggaaaaaga-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pERB257 was a gift from Michael Lampson (Addgene plasmid # 67761 ; http://n2t.net/addgene:67761 ; RRID:Addgene_67761)

  • For your References section:

    Optogenetic control of organelle transport using a photocaged chemical inducer of dimerization. Ballister ER, Ayloo S, Chenoweth DM, Lampson MA, Holzbaur EL. Curr Biol. 2015 May 18;25(10):R407-8. doi: 10.1016/j.cub.2015.03.056. 10.1016/j.cub.2015.03.056 PubMed 25989077
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