pWalium-ChtVis-Tomato
(Plasmid #67756)

• Purpose: Expresses a fluorescent chitin reporter under Gal4 control in Drosophila.
• Depositing Lab: Paul Adler
• Publication: Sobala et al Development. 2015 Nov 15;142(22):3974-81. doi: 10.1242/dev.126987. Epub 2015 Sep 22.

Backbone

• Vector backbone: pWalium-moe
• Backbone manufacturer: Norbert Perrimon
• Backbone size w/o insert (bp): 7541
• Total vector size (bp): 11852
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dyl - chitin binding domain - tdTomato - dyl
• Alt name: Chitinase A1
• Species: D. melanogaster (fly); Bacillus circulans
• Tag / Fusion Protein:
  • td-tomato (C terminal on insert)

Resource Information

• Supplemental Documents:
  • genbank file format
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid contains pieces of DNA from a number of different sources. The td-Tomato was obtained from FUtdTW, Addgene.
Chitin binding domain was purchased from New England Biolabs
The dyl cDNA came from the Drosophila genome project
The pWalium10 moe vector came originally from the N. Perrimon Lab.