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Addgene

pWalium-ChtVis-Tomato
(Plasmid #67756)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67756 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pWalium-moe
  • Backbone manufacturer
    Norbert Perrimon
  • Backbone size w/o insert (bp) 7541
  • Total vector size (bp) 11852
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dyl - chitin binding domain - tdTomato - dyl
  • Alt name
    Chitinase A1
  • Species
    D. melanogaster (fly); Bacillus circulans
  • Tag / Fusion Protein
    • td-tomato (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid contains pieces of DNA from a number of different sources. The td-Tomato was obtained from FUtdTW, Addgene.
Chitin binding domain was purchased from New England Biolabs
The dyl cDNA came from the Drosophila genome project
The pWalium10 moe vector came originally from the N. Perrimon Lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWalium-ChtVis-Tomato was a gift from Paul Adler (Addgene plasmid # 67756 ; http://n2t.net/addgene:67756 ; RRID:Addgene_67756)
  • For your References section:

    ChtVis-Tomato, a genetic reporter for in vivo visualization of chitin deposition in Drosophila. Sobala LF, Wang Y, Adler PN. Development. 2015 Nov 15;142(22):3974-81. doi: 10.1242/dev.126987. Epub 2015 Sep 22. 10.1242/dev.126987 PubMed 26395478