Skip to main content
Addgene

pETCON-TNF
(Plasmid #67748)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67748 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pETCON
  • Backbone size w/o insert (bp) 6133
  • Total vector size (bp) 6676
  • Modifications to backbone
    Subcloned using NdeI/XhoI sites
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    TRP1, LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TNF-Alpha
  • Species
    Synthetic
  • Insert Size (bp)
    543

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETCON-TNF was a gift from Timothy Whitehead (Addgene plasmid # 67748 ; http://n2t.net/addgene:67748 ; RRID:Addgene_67748)

  • For your References section:

    Rapid fine conformational epitope mapping using comprehensive mutagenesis and deep sequencing. Kowalsky CA, Faber MS, Nath A, Dann HE, Kelly VW, Liu L, Shanker P, Wagner EK, Maynard JA, Chan C, Whitehead TA. J Biol Chem. 2015 Oct 30;290(44):26457-70. doi: 10.1074/jbc.M115.676635. Epub 2015 Aug 20. 10.1074/jbc.M115.676635 PubMed 26296891
Related items:
Commonly requested with: