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Addgene

pETCON-PTX-S1-220
(Plasmid #67747)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67747 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETCON
  • Backbone size w/o insert (bp) 6142
  • Total vector size (bp) 6805
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    TRP1, LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTxS1-220
  • Species
    Bordella Pertussis
  • Insert Size (bp)
    663

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETCON-PTX-S1-220 was a gift from Timothy Whitehead (Addgene plasmid # 67747 ; http://n2t.net/addgene:67747 ; RRID:Addgene_67747)

  • For your References section:

    Rapid fine conformational epitope mapping using comprehensive mutagenesis and deep sequencing. Kowalsky CA, Faber MS, Nath A, Dann HE, Kelly VW, Liu L, Shanker P, Wagner EK, Maynard JA, Chan C, Whitehead TA. J Biol Chem. 2015 Oct 30;290(44):26457-70. doi: 10.1074/jbc.M115.676635. Epub 2015 Aug 20. 10.1074/jbc.M115.676635 PubMed 26296891
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