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Addgene

pfMDH_R373A
(Plasmid #67731)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67731 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5232
  • Total vector size (bp) 6714
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
  • Alt name
    pfMDH
  • Alt name
    mtlD
  • Species
    Pseudomonas fluorescens
  • Insert Size (bp)
    1485
  • Mutation
    Changed arginine 373 to alanine
  • GenBank ID
    AAC04472.1
  • Promoter T7
  • Tag / Fusion Protein
    • 6x-His tag (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pfMDH_R373A was a gift from Justin Siegel (Addgene plasmid # 67731 ; http://n2t.net/addgene:67731 ; RRID:Addgene_67731)

  • For your References section:

    Quantitative functional characterization of conserved molecular interactions in the active site of mannitol 2-dehydrogenase. Lucas JE, Siegel JB. Protein Sci. 2015 Jun;24(6):936-45. doi: 10.1002/pro.2669. Epub 2015 Apr 2. 10.1002/pro.2669 PubMed 25752240
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