pME-secAnxaV-NS
(Plasmid
#67718)
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PurposeGateway entry clone containing secreted human AnxaV with no stop codon
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67718 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR 221
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesecAnxaV-NS
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SpeciesSynthetic
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-secAnxaV-NS was a gift from Rob Parton (Addgene plasmid # 67718 ; http://n2t.net/addgene:67718 ; RRID:Addgene_67718) -
For your References section:
In vivo characterization of microglial engulfment of dying neurons in the zebrafish spinal cord. Morsch M, Radford R, Lee A, Don EK, Badrock AP, Hall TE, Cole NJ, Chung R. Front Cell Neurosci. 2015 Aug 31;9:321. doi: 10.3389/fncel.2015.00321. eCollection 2015. 10.3389/fncel.2015.00321 PubMed 26379496
Map uploaded by the depositor.
