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Addgene

pTmaM4
(Plasmid #67714)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67714 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5708
  • Total vector size (bp) 6000
  • Modifications to backbone
    Insertion between NcoI and BamHI sites
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Thermotoga marítima Ffh-M domain
  • Species
    Thermotoga marítima
  • Insert Size (bp)
    500
  • Promoter T7 RNA polymerase
  • Tag / Fusion Protein
    • hexahistidine tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTmaM4 was a gift from Robert Batey & Jeffrey Kieft (Addgene plasmid # 67714 ; http://n2t.net/addgene:67714 ; RRID:Addgene_67714)

  • For your References section:

    A general method for rapid and nondenaturing purification of RNAs. Kieft JS, Batey RT. RNA. 2004 Jun;10(6):988-95. PubMed 15146082
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