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Addgene

pCS-Fv2Eflt4um200-2a-tdTomato
(Plasmid #67698)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67698 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSDest2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fv2E-flt4-2A-tdTomato
  • Species
    D. rerio (zebrafish)
  • Mutation
    um200
  • Entrez Gene
    flt4 (a.k.a. cb1059)
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • 2A-tdTomato (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS-Fv2Eflt4um200-2a-tdTomato was a gift from Nathan Lawson (Addgene plasmid # 67698 ; http://n2t.net/addgene:67698 ; RRID:Addgene_67698)

  • For your References section:

    Vegfc acts through ERK to induce sprouting and differentiation of trunk lymphatic progenitors. Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND. Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12. 10.1242/dev.137901 PubMed 27621059
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