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Addgene

pME-Fv2E-flt4-ns
(Plasmid #67695)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67695 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fv2E-flt4
  • Mutation
    no stop codon; D1302E (see depositor's comment below)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The D1302E mutation in flt4 does not affect protein function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-Fv2E-flt4-ns was a gift from Nathan Lawson (Addgene plasmid # 67695 ; http://n2t.net/addgene:67695 ; RRID:Addgene_67695)

  • For your References section:

    Vegfc acts through ERK to induce sprouting and differentiation of trunk lymphatic progenitors. Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND. Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12. 10.1242/dev.137901 PubMed 27621059
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