pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC
(Plasmid
#67687)
-
PurposeUAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassette
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67687 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepmTol-InsUAS-Dest
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namedusp6
-
SpeciesD. rerio (zebrafish)
-
MutationS159A, S196A
-
Entrez Genedusp6 (a.k.a. Mkp3, wu:fd99d09)
-
Tag
/ Fusion Protein
- 6xmyc-NLS-tdTomato-2A (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
tdTomato has mutation G172S and this does not affect protein function or fluorescence
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC was a gift from Nathan Lawson (Addgene plasmid # 67687 ; http://n2t.net/addgene:67687 ; RRID:Addgene_67687) -
For your References section:
Vegfa signals through ERK to promote angiogenesis, but not artery differentiation. Shin M, Beane TJ, Quillien A, Male I, Zhu LJ, Lawson ND. Development. 2016 Oct 15;143(20):3796-3805. Epub 2016 Aug 30. 10.1242/dev.137919 PubMed 27578780
