pME-6MTnlstdTomato2A
(Plasmid
#67685)
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PurposeMiddle entry nuclear localized tdTomato reporter with 6xMT and 2A
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67685 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametdTomato
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Tags
/ Fusion Proteins
- 6xMyc-NLS (N terminal on insert)
- 2A (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-6MTnlstdTomato2A was a gift from Nathan Lawson (Addgene plasmid # 67685 ; http://n2t.net/addgene:67685 ; RRID:Addgene_67685) -
For your References section:
Vegfa signals through ERK to promote angiogenesis, but not artery differentiation. Shin M, Beane TJ, Quillien A, Male I, Zhu LJ, Lawson ND. Development. 2016 Oct 15;143(20):3796-3805. Epub 2016 Aug 30. 10.1242/dev.137919 PubMed 27578780
