p3E-Dusp6DM_S159/197A
(Plasmid
#67684)
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Purpose3' entry clone with activated Dusp6 (S159/167A)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 67684 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR-P2-P3
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Backbone manufacturerinvitrogen
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedusp6
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SpeciesD. rerio (zebrafish)
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MutationS159A, S197A
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Entrez Genedusp6 (a.k.a. Mkp3, wu:fd99d09)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3E-Dusp6DM_S159/197A was a gift from Nathan Lawson (Addgene plasmid # 67684 ; http://n2t.net/addgene:67684 ; RRID:Addgene_67684) -
For your References section:
Vegfa signals through ERK to promote angiogenesis, but not artery differentiation. Shin M, Beane TJ, Quillien A, Male I, Zhu LJ, Lawson ND. Development. 2016 Oct 15;143(20):3796-3805. Epub 2016 Aug 30. 10.1242/dev.137919 PubMed 27578780