pFA6a SVEM hphMX6 lys1-I-SceI cleavage site
(Plasmid
#67648)
-
PurposeI-SceI cleavage site at lys1-hphMX6
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 67648 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepFA6a
- Total vector size (bp) 5014
-
Vector typeto test I-SceI cleavage efficiency
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert 1
-
Gene/Insert namelys1
-
SpeciesS. pombe (fission yeast)
-
Entrez Genelys1 (a.k.a. SPAP7G5.04c)
Gene/Insert 2
-
Gene/Insert namehphMX6
-
Alt namehygromycin resistance gene
-
SpeciesSynthetic
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pFA6a SVEM hphMX6 lys1-I-SceI cleavage site was a gift from Kurt Runge (Addgene plasmid # 67648 ; http://n2t.net/addgene:67648 ; RRID:Addgene_67648) -
For your References section:
A Heterochromatin Domain Forms Gradually at a New Telomere and Is Dynamic at Stable Telomeres. Wang J, Eisenstatt JR, Audry J, Cornelius K, Shaughnessy M, Berkner KL, Runge KW. Mol Cell Biol. 2018 Jul 16;38(15). pii: MCB.00393-17. doi: 10.1128/MCB.00393-17. Print 2018 Aug 1. 10.1128/MCB.00393-17 PubMed 29784772