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Addgene

pJH001
(Plasmid #67641)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67641 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSII425
  • Backbone size w/o insert (bp) 6841
  • Total vector size (bp) 12366
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cas9
  • Species
    S. cerevisiae (budding yeast); Streptococcus pyogenes
  • Insert Size (bp)
    4151

Gene/Insert 2

  • Gene/Insert name
    Empty

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cas9 gene cloned from pTDH3-dCas9 and mutated extensively

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH001 was a gift from John Wyrick (Addgene plasmid # 67641 ; http://n2t.net/addgene:67641 ; RRID:Addgene_67641)

  • For your References section:

    New vectors for simple and streamlined CRISPR-Cas9 genome editing in Saccharomyces cerevisiae. Laughery MF, Hunter T, Brown A, Hoopes J, Ostbye T, Shumaker T, Wyrick JJ. Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 21. 10.1002/yea.3098 PubMed 26305040
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