pT040
(Plasmid #67640)

• Purpose: URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette
• Depositing Lab: John Wyrick
• Publication: Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 21.

Backbone

• Vector backbone: pCT3
• Backbone size w/o insert (bp): 4749
• Total vector size (bp): 5187
• Vector type: Yeast Expression, CRISPR
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Need to purify DNA from Dam- E.coli strain for efficient BclI cutting
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: single guide RNA expression cassette
• gRNA/shRNA sequence: target sequence into cassette is cloned using BclI-SwaI restriction enzymes
• Species: S. cerevisiae (budding yeast), Synthetic
• Promoter: pSNR52

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacII (not destroyed)
• 3′ cloning site: EagI (not destroyed)
• 5′ sequencing primer: T3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry