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mRFP-dmBACCS2
(Plasmid #67630)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67630 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc5.1/V5-His A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5362
  • Total vector size (bp) 7643
  • Vector type
    Drosophila expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mRFP-dmBACCS2
  • Species
    D. melanogaster (fly); Avena sativa (oat)
  • Insert Size (bp)
    2281
  • Promoter Drosophila actin gene 5C gene promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer AC5
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mRFP-dmBACCS2 was a gift from Takao Nakata (Addgene plasmid # 67630 ; http://n2t.net/addgene:67630 ; RRID:Addgene_67630)

  • For your References section:

    Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS. Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata T. Nat Commun. 2015 Aug 18;6:8021. doi: 10.1038/ncomms9021. 10.1038/ncomms9021 PubMed 26282514
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