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pRS315-0nt-telo-I-SceI
(Plasmid #67624)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67624 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS315
  • Vector type
    yeast cen/ars plasmid
  • Selectable markers
    Hygromycin, LEU2, URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    3' gal1 unique sequence
  • Species
    S. pombe (fission yeast)

Gene/Insert 2

  • Gene/Insert name
    ura4
  • Species
    S. pombe (fission yeast)
  • Entrez Gene
    ura4 (a.k.a. SPCC330.05c)

Gene/Insert 3

  • Gene/Insert name
    telomere cassette and I-SceI cut site

Gene/Insert 4

  • Gene/Insert name
    hph

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS315-0nt-telo-I-SceI was a gift from Kurt Runge (Addgene plasmid # 67624 ; http://n2t.net/addgene:67624 ; RRID:Addgene_67624)

  • For your References section:

    A Heterochromatin Domain Forms Gradually at a New Telomere and Is Dynamic at Stable Telomeres. Wang J, Eisenstatt JR, Audry J, Cornelius K, Shaughnessy M, Berkner KL, Runge KW. Mol Cell Biol. 2018 Jul 16;38(15). pii: MCB.00393-17. doi: 10.1128/MCB.00393-17. Print 2018 Aug 1. 10.1128/MCB.00393-17 PubMed 29784772
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