pa-G-GECO1.2
(Plasmid #67622)

• Purpose: photoactivatable fluorescent calcium reporter
• Depositing Lab: Ehud Isacoff
• Publication: Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.

Backbone

• Vector backbone: pCMV
• Backbone manufacturer: Campbell RE
• Backbone size w/o insert (bp): 3200
• Total vector size (bp): 4457
• Modifications to backbone: Customized Vector, see Addgene # 32446, Zhao et al Science. 2011.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pa-G-GECO1.2
• Alt name: photoactivatable G-GECO1.2
• Species: Synthetic
• Insert Size (bp): 1257
• Mutation: V81H, M84K, L189F, T190S

Resource Information

• A portion of this plasmid was derived from a plasmid made by: clone purchased from Addgene, engineered by Robert Campbell. Publication: Zhao et al Science. 2011 Sep 8.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry