pLV hUbC-dCas9 KRAB-T2A-GFP
(Plasmid #67620)

• Purpose: Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP
• Depositing Lab: Charles Gersbach
• Publication: Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku749.

Backbone

• Vector backbone: FUGW
• Total vector size (bp): 15000
• Vector type: Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: humanized dead Cas9 KRAB T2A GFP
• Species: S. Pyogenes
• Mutation: D10A and H840A
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry