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Purpose(Empty Backbone) A pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technology
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67616 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePCS2+ was modified to generate pCSf107mT
- Backbone size (bp) 4181
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Modifications to backboneinserted a PCR product that facilitates GATEWAY insertions behind an in frame 3'HA tag
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Vector typeMammalian Expression ; in vitro transcription or translation off of an SP6 promoter, Xenopus expression
- Promoter CMV and SP6
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Tag
/ Fusion Protein
- three inframe 3' HA tags (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCSF107mT-GATEWAY-3'-3HA was a gift from Todd Stukenberg (Addgene plasmid # 67616 ; http://n2t.net/addgene:67616 ; RRID:Addgene_67616) -
For your References section:
The Xenopus ORFeome: A resource that enables functional genomics. Grant IM, Balcha D, Hao T, Shen Y, Trivedi P, Patrushev I, Fortriede JD, Karpinka JB, Liu L, Zorn AM, Stukenberg PT, Hill DE, Gilchrist MJ. Dev Biol. 2015 Dec 15;408(2):345-57. doi: 10.1016/j.ydbio.2015.09.004. Epub 2015 Sep 29. 10.1016/j.ydbio.2015.09.004 PubMed 26391338
Map uploaded by the depositor.
