pFA-LEU2-I-SceI
(Plasmid #67595)

• Purpose: I-SceI expression regulated by tetracycline inducible system in S. pombe
• Depositing Lab: Kurt Runge
• Publication: Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii: MCB.00393-17. doi: 10.1128/MCB.00393-17. Print 2018 Aug 1.

Backbone

• Vector backbone: pFA-LEU2
• Backbone size w/o insert (bp): 5830
• Total vector size (bp): 9786
• Vector type: Gene Targeting in yeast
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: nmt terminator
• Species: S. pombe (fission yeast), Synthetic
• Insert Size (bp): 1100

Gene/Insert 2

• Gene/Insert name: I-SceI
• Species: S. pombe (fission yeast), Synthetic
• Insert Size (bp): 900

Gene/Insert 3

• Gene/Insert name: TetR
• Species: S. pombe (fission yeast), Synthetic
• Insert Size (bp): 2200

Resource Information

• Supplemental Documents:
  • pFA-LEU2-I-SceI

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry