FLAG-RFP-UCR1C
(Plasmid #67582)

• Purpose: Expresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cells
• Depositing Lab: Graeme Carnegie
• Publication: Wang et al Cell Signal. 2015 May;27(5):908-22. doi: 10.1016/j.cellsig.2015.02.003. Epub 2015 Feb 12.

Backbone

• Vector backbone: pFLAG-Cerulean
• Modifications to backbone: Deletion of Cerulean Addition of TagRFP
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: C-terminus of upstream conserved region 1 of phosphodiesterase 4D3
• Alt name: PDE4D3 UCR1
• Species: H. sapiens (human)
• Mutation: Amino acid residues 80-136 of PDE4D3
• Entrez Gene: PDE4D (a.k.a. ACRDYS2, DPDE3, HSPDE4D, PDE43, PDE4DN2, STRK1)
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • TagRFP (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry