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Addgene

FLAG-Cerulean-UCR1
(Plasmid #67578)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67578 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFLAG-Cerulean
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Upstream conserved region 1 of phosphodiesterase 4D3
  • Alt name
    PDE4D3 UCR1
  • Species
    H. sapiens (human)
  • Mutation
    Amino acid residues 17-136 of PDE4D3
  • Entrez Gene
    PDE4D (a.k.a. ACRDYS2, DPDE3, HSPDE4D, PDE43, PDE4DN2, STRK1)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • Cerulean (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-Cerulean-UCR1 was a gift from Graeme Carnegie (Addgene plasmid # 67578 ; http://n2t.net/addgene:67578 ; RRID:Addgene_67578)

  • For your References section:

    UCR1C is a novel activator of phosphodiesterase 4 (PDE4) long isoforms and attenuates cardiomyocyte hypertrophy. Wang L, Burmeister BT, Johnson KR, Baillie GS, Karginov AV, Skidgel RA, O'Bryan JP, Carnegie GK. Cell Signal. 2015 May;27(5):908-22. doi: 10.1016/j.cellsig.2015.02.003. Epub 2015 Feb 12. 10.1016/j.cellsig.2015.02.003 PubMed 25683917
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