sspa-GCaMP6m
(Plasmid #67560)

• Purpose: short photoactivatable fluorescent calcium reporter
• Depositing Lab: Ehud Isacoff
• Publication: Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.

Backbone

• Vector backbone: pGP-CMV (pEGFP-N1)
• Backbone manufacturer: Clonetech
• Backbone size w/o insert (bp): 3955
• Total vector size (bp): 5308
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sspa-GCaMP6m
• Alt name: short superfolder photoactivatable GCaMP6f
• Species: Synthetic
• Insert Size (bp): 1261
• Mutation: K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196N, L221F, T222S, I250V, F256S, N262T

Resource Information

• A portion of this plasmid was derived from a plasmid made by: derived from Addgene clone #40754, published by Chen TW et al. article: ultrasensitive fluorescent proteins for imaging neuronal activity doi: 10.1038/nature12354

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry