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Addgene

spa-GCaMP6f
(Plasmid #67558)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67558 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGP-CMV (pEGFP-N1)
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 3955
  • Total vector size (bp) 5308
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    spa-GCaMP6f
  • Alt name
    superfolder photoactivatable GCaMP6f
  • Species
    Synthetic
  • Insert Size (bp)
    1353
  • Mutation
    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196N, L221F, T222S, I250V, F256S, N262T
  • Promoter CMV
  • Tag / Fusion Protein
    • HIS-tag (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer SV40 reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    derived from Addgene clone #40755, published by Chen TW et al. article: ultrasensitive fluorescent proteins for imaging neuronal activity doi: 10.1038/nature12354

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    spa-GCaMP6f was a gift from Ehud Isacoff (Addgene plasmid # 67558 ; http://n2t.net/addgene:67558 ; RRID:Addgene_67558)

  • For your References section:

    Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging. Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, Rockwell NC, Martin SS, Lagarias JC, Isacoff EY. Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480. 10.1038/nmeth.3480 PubMed 26167640
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