pCfB2228
(Plasmid
#67540)
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Purpose(Empty Backbone) EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistance
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67540 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneEasyClone
- Backbone size (bp) 5912
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Vector typeYeast Expression, Cre/Lox ; integrative
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCfB2228 was a gift from Irina Borodina (Addgene plasmid # 67540 ; http://n2t.net/addgene:67540 ; RRID:Addgene_67540) -
For your References section:
EasyClone 2.0: expanded toolkit of integrative vectors for stable gene expression in industrial Saccharomyces cerevisiae strains. Stovicek V, Borja GM, Forster J, Borodina I. J Ind Microbiol Biotechnol. 2015 Sep 16. 10.1007/s10295-015-1684-8 PubMed 26376869
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Map uploaded by the depositor.
