pcDNA3 AIF 1-90 mCherry FRB
(Plasmid #67530)

• Purpose: For expression of mCherry fused FRB to the mitochondrial innermembrane
• Depositing Lab: Stephen Tait
• Publication: Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi: 10.1016/j.molcel.2015.01.018. Epub 2015 Feb 19.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 6687
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: AIF a.a. 1 - 90
• Species: H. sapiens (human)
• Insert Size (bp): 270
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (unknown if destroyed)
• 3′ cloning site: Kpn I (unknown if destroyed)
• 5′ sequencing primer: CMV

Gene/Insert 2

• Gene/Insert name: mCherry
• Promoter: CMV
• Tags / Fusion Proteins:
  • AIF 1 - 90 (N terminal on insert)
  • FRB (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn 1 (unknown if destroyed)
• 3′ cloning site: Not 1 (unknown if destroyed)
• 5′ sequencing primer: CMV

Gene/Insert 3

• Gene/Insert name: FRB
• Mutation: T->L
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information for Gene/Insert 3

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bam H1 (unknown if destroyed)
• 3′ cloning site: Xho I (unknown if destroyed)
• 5′ sequencing primer: CMV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry