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Addgene

pAAV-EF1a-tdTomato-WPRE-pGHpA
(Plasmid #67527)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67527 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
  • Backbone manufacturer
    karl deisseroth
  • Total vector size (bp) 6775
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tandem dimer tomato
  • Alt name
    tdTomato
  • Insert Size (bp)
    1428
  • Promoter EF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1a-tdTomato-WPRE-pGHpA was a gift from Botond Roska (Addgene plasmid # 67527 ; http://n2t.net/addgene:67527 ; RRID:Addgene_67527)

  • For your References section:

    PRESYNAPTIC NETWORKS. Single-cell-initiated monosynaptic tracing reveals layer-specific cortical network modules. Wertz A, Trenholm S, Yonehara K, Hillier D, Raics Z, Leinweber M, Szalay G, Ghanem A, Keller G, Rozsa B, Conzelmann KK, Roska B. Science. 2015 Jul 3;349(6243):70-4. doi: 10.1126/science.aab1687. 10.1126/science.aab1687 PubMed 26138975
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