pAAV-EF1a-GCaMP6s-WPRE-pGHpA
(Plasmid #67526)

• Purpose: "Fluorescent reporter for calcium imaging"
• Depositing Lab: Botond Roska
• Publication: Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi: 10.1126/science.aab1687.

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• Serotype: Select serotype for details See details about
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Backbone

• Vector backbone: pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
• Backbone manufacturer: karl deisseroth
• Total vector size (bp): 6680
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GCaMP6s
• Species: A. victoria (jellyfish)
• Insert Size (bp): 1350
• Promoter: EF1a
• Tags / Fusion Proteins:
  • 6xHis (N terminal on insert)
  • T7 tag (N terminal on insert)
  • Xpress epitope (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: EF1a-F
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Douglas Kim
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry