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pAAV-EF1a-GCaMP6s-WPRE-pGHpA
(Plasmid #67526)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67526 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
  • Backbone manufacturer
    karl deisseroth
  • Total vector size (bp) 6680
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GCaMP6s
  • Species
    A. victoria (jellyfish)
  • Insert Size (bp)
    1350
  • Promoter EF1a
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • T7 tag (N terminal on insert)
    • Xpress epitope (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Douglas Kim
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1a-GCaMP6s-WPRE-pGHpA was a gift from Botond Roska (Addgene plasmid # 67526 ; http://n2t.net/addgene:67526 ; RRID:Addgene_67526)

  • For your References section:

    PRESYNAPTIC NETWORKS. Single-cell-initiated monosynaptic tracing reveals layer-specific cortical network modules. Wertz A, Trenholm S, Yonehara K, Hillier D, Raics Z, Leinweber M, Szalay G, Ghanem A, Keller G, Rozsa B, Conzelmann KK, Roska B. Science. 2015 Jul 3;349(6243):70-4. doi: 10.1126/science.aab1687. 10.1126/science.aab1687 PubMed 26138975
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