pSuper-PICK1 shRNA
(Plasmid
#67522)
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PurposeExpresses shRNA against PICK1
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67522 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSuper
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Backbone manufacturerOligoengine
- Backbone size w/o insert (bp) 3176
- Total vector size (bp) 3213
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Vector typeMammalian Expression, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer AATACGACTCACTATAG
- 3′ sequencing primer CTTTAGTGAGGGTTAAT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSuper-PICK1 shRNA was a gift from Victor Anggono & Richard Huganir (Addgene plasmid # 67522 ; http://n2t.net/addgene:67522 ; RRID:Addgene_67522) -
For your References section:
PICK1 loss of function occludes homeostatic synaptic scaling. Anggono V, Clem RL, Huganir RL. J Neurosci. 2011 Feb 9;31(6):2188-96. doi: 10.1523/JNEUROSCI.5633-10.2011. 10.1523/JNEUROSCI.5633-10.2011 PubMed 21307255
Map uploaded by the depositor.
