Skip to main content
Addgene

cryS96
(Plasmid #67519)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67519 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET25b (+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5547
  • Total vector size (bp) 7053
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mini-αB-crystallin fused to ELP S96 (VPGSG)
  • Alt name
    cry, mini-αB-crystallin
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1506
  • Mutation
    mini-αB-crystallin contains residues 73–92 of aB-crystallin
  • Entrez Gene
    CRYAB (a.k.a. CMD1II, CRYA2, CTPP2, CTRCT16, HEL-S-101, HSPB5, MFM2)
  • Promoter T7
  • Tag / Fusion Protein
    • elastin-like polypeptide (ELP) S96 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cryS96 was a gift from Andrew MacKay (Addgene plasmid # 67519 ; http://n2t.net/addgene:67519 ; RRID:Addgene_67519)

  • For your References section:

    Protein polymer nanoparticles engineered as chaperones protect against apoptosis in human retinal pigment epithelial cells. Wang W, Sreekumar PG, Valluripalli V, Shi P, Wang J, Lin YA, Cui H, Kannan R, Hinton DR, MacKay JA. J Control Release. 2014 Oct 10;191:4-14. doi: 10.1016/j.jconrel.2014.04.028. Epub 2014 Apr 26. 10.1016/j.jconrel.2014.04.028 PubMed 24780268
Related items:
Commonly requested with: