PM-FRB-CFP
(Plasmid #67517)

• Purpose: FRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)
• Depositing Lab: Tamas Balla
• Publication: Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Backbone

• Vector backbone: CFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mTOR
• Species: H. sapiens (human)
• Mutation: FRB domain only (residues 2019–2114)
• Entrez Gene: MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
• Promoter: CMV
• Tags / Fusion Proteins:
  • PM tethering sequence (MLCCMRRTKQVEKNDDDQKI) from human GAP43 (residues 1–20) (N terminal on insert)
  • HA (C terminal on insert)
  • CFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry