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mRFP-FKBP12-5ptpase domain
(Plasmid #67516)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67516 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mRFPC1

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FKBP12-5ptpase enzyme
  • Alt name
    INPP5E (aa214–644)
  • Species
    H. sapiens (human)
  • Mutation
    INPP5E has C641A to destroy the C-terminal CAAX domain
  • Entrez Gene
    INPP5E (a.k.a. CORS1, CPD4, JBTS1, MORMS, PPI5PIV, pharbin)
  • Entrez Gene
    FKBP1A (a.k.a. FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE)
  • Tag / Fusion Protein
    • mRFP (N terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mRFP-FKBP12-5ptpase domain was a gift from Tamas Balla (Addgene plasmid # 67516 ; http://n2t.net/addgene:67516 ; RRID:Addgene_67516)

  • For your References section:

    Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells. Varnai P, Thyagarajan B, Rohacs T, Balla T. J Cell Biol. 2006 Nov 6;175(3):377-82. 10.1083/jcb.200607116 PubMed 17088424
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