pCAGGS-FuG-E
(Plasmid #67509)

• Purpose: This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E envelope coating virus particle with NeuRet.
• Depositing Lab: Kazuto Kobayashi
• Publication: Kato et al J Neurosci Methods. 2014 Apr 30;227:151-8. doi: 10.1016/j.jneumeth.2014.02.015. Epub 2014 Mar 5.

Backbone

• Vector backbone: pCAGGS vector
• Backbone size w/o insert (bp): 4900
• Total vector size (bp): 6406
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: DH5alpha is also a suitable growth strain.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fusion Glycoprotein type E
• Alt name: FuG-E
• Species: G. gallus (chicken), B. taurus (bovine); Vesicular stomatitis Indiana virus, Rabies virus, Cytomegalovirus, Simian virus 40, Oryctolagus cuniculus
• Insert Size (bp): 1515
• Promoter: CAGGS
• Tag / Fusion Protein:
  • Fusion protein of Vesicular stomatitis Indiana virus and Rabies virus (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Unknown
• 3′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • Sequence data
• A portion of this plasmid was derived from a plasmid made by: Fusion glycoproteins are composed of parts of rabies virus glycoprotein (RV-G) and vesicular stomatitis virus glycoprotein (VSV-G). In three glycoproteins including FuG-B, FuG-C, and FuG-E, the sequences of RV-G are derived from the challenged virus standard (CVS) strain of rabies virus, whereas those in FuG-B2 are from Pasteur virus (PV) strain of the virus. The CVS RV-G cDNA was provided by Dr. K. Morimoto at Yasuda Women’s University (Hiroshima, Japan) (ref: MORIMOTO, K., HOOPER, D.C., CARBAUGH, H., FU, Z.F., KOPROWSKI, H., and DIETZSCHOLD, B. Rabies virus quasispecies: implications for pathogenesis. Proc. Natl. Acad. Sci. USA 95, 3152-3156, 1998), and the PV RV-G cDNA was chemically synthesized on the basis of the GenBank data (accession number: GU992322). The VSV-G cDNA was provided by Dr. A. Nienhuis at St. Jude Children’s Research Hospital.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry