pBSM
(Plasmid
#67505)
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PurposeExpress the tRNAMet scaffold
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67505 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBSTNAV
- Backbone size w/o insert (bp) 3019
- Total vector size (bp) 3080
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemodified tRNAMet
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Insert Size (bp)74
- Promoter lpp
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBSM was a gift from Luc Ponchon (Addgene plasmid # 67505 ; http://n2t.net/addgene:67505 ; RRID:Addgene_67505) -
For your References section:
A generic protocol for the expression and purification of recombinant RNA in Escherichia coli using a tRNA scaffold. Ponchon L, Beauvais G, Nonin-Lecomte S, Dardel F. Nat Protoc. 2009;4(6):947-59. doi: 10.1038/nprot.2009.67. Epub 2009 May 28. 10.1038/nprot.2009.67 PubMed 19478810