pSUPER-ARF4b
(Plasmid
#67498)
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PurposeExpression of shRNA targeting human ARF4b
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67498 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSUPER
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Vector typeMammalian Expression, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHsARF4 shRNA
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Alt namepSUPER-ARF4b
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gRNA/shRNA sequenceTCACTGATGGCCATAGCAT
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SpeciesH. sapiens (human)
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Entrez GeneARF4 (a.k.a. ARF2)
- Promoter H1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer M13-Fwd
- 3′ sequencing primer M13-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Sequence deviates from pSUPER4b described in Volpicelli-Daley but still targets to HsARF4 ORF
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSUPER-ARF4b was a gift from Richard Kahn (Addgene plasmid # 67498 ; http://n2t.net/addgene:67498 ; RRID:Addgene_67498) -
For your References section:
Isoform-selective effects of the depletion of ADP-ribosylation factors 1-5 on membrane traffic. Volpicelli-Daley LA, Li Y, Zhang CJ, Kahn RA. Mol Biol Cell. 2005 Oct;16(10):4495-508. Epub 2005 Jul 19. 10.1091/mbc.e04-12-1042 PubMed 16030262