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Addgene

pSUPER-ARF1b
(Plasmid #67494)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67494 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUPER
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HsARF1 shRNA
  • Alt name
    pSUPER-ARF1b
  • gRNA/shRNA sequence
    TGACAGAGAGCGTGTGAAC
  • Species
    H. sapiens (human)
  • Entrez Gene
    ARF1 (a.k.a. PVNH8)
  • Promoter H1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer M13-Fwd
  • 3′ sequencing primer M13-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUPER-ARF1b was a gift from Richard Kahn (Addgene plasmid # 67494 ; http://n2t.net/addgene:67494 ; RRID:Addgene_67494)

  • For your References section:

    Isoform-selective effects of the depletion of ADP-ribosylation factors 1-5 on membrane traffic. Volpicelli-Daley LA, Li Y, Zhang CJ, Kahn RA. Mol Biol Cell. 2005 Oct;16(10):4495-508. Epub 2005 Jul 19. 10.1091/mbc.e04-12-1042 PubMed 16030262
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